ABSTRACT
Based on the combination of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF) and Waters UNIFI software, the chemical constituents of the classic prescription Xiaochengqi Decoction were qualitatively analyzed and identified. The UPLC conditions are as follows: Acquity HSS T3 reverse phase column(2.1 mm ×100 mm, 1.8 μm), column temperature of 30 ℃, mobile phase of 0.1% formic acid aqueous solution(A)-acetonitrile(B), and flow rate of 0.3 mL·min~(-1). High-resolution MS data of Xiaochengqi Decoction were collected in ESI~(+/-) modes by Fast DDA. The structures of the chemical constituents were tentatively characterized or identified by UNIFI software according to the retention time of reference standards and characteristic fragment ions in MS profile, and literature data. A total of 233 components in Xiaochengqi Decoction were identified, with 93 from wine-processed Rhei Radix et Rhizoma, 104 from bran-processed Aurantii Fructus Immaturus, and 36 from ginger-processed Magnoliae Officinalis Cortex. These 233 components included anthraquinones, flavonoids, lignans, alkaloids, coumarins, and phenylethanoid glycosides. The result provided experimental evidence for the further study on establishment of quality standard and product development of the formula.
Subject(s)
Chromatography, High Pressure Liquid/methods , DDT/analogs & derivatives , Drugs, Chinese Herbal/chemistry , Mass Spectrometry , Rhizome/chemistry , SoftwareABSTRACT
Objective:To optimize the decoction process of Xiaochengqi decoction by Box-Behnken response surface method. Methods:Rhein,aloe-emodin and synephrine were chosen as the evaluation indices. After the material-liquid ratio, decocting frequency and decocting time were studied,Box-Behnken response surface method was used to optimize the three factors using the weighted calculation value of the extraction rate of above indicator components. Results:The best conditions were as follows:the material-liquid ratio was 1:10,the decocting frequency was 3,and the decocting time was 1.0 h. The deviation of measured value and predicted value was 1.15%. Conclusion:The best decocting conditions are promising and reliable.
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Objective To explore the establishing methods and differences of rat models of spleen deficiency and spleen deficiency liver cancer using the traditional Chinese medicine Dachengqi and Xiaochengqi decoctions .Methods Spleen-deficiency rat models were developed by multifactor methods:bitter-cold purgation ( Dachengqi or Xiaochengqi de-coction), cold-wet environment, tiredness, and fasting on alternate days for 30 days.Seven days after spleen-deficiency modeled,liver cancer in the spleen-deficiency rats and normal rats was developed by subcutaneously inoculation of Walker -256 carcinoma cell line in nude mice and then transplanted into rat livers .Liver cancer models were observed for 35 days. Sixty 3-week old male Wistar rats were randomly distributed into 4 groups: normal group , liver cancer model group , and Dachengqi and Xiaochengqi decoction groups .Degree of spleen deficiency , changes of the body-weight, survival time and tumor formation were recorded .Results Spleen deficiency rat models were successfully established .The weight gain of rats in the spleen-deficiency groups was significantly inhibited (P<0.01), and during the first 20 days (but not later) the average body weight of the Dachengqi decoction group was significantly higher than that of the Xiaochengqi decoction group (P<0.05).Spleen-deficiency scores of rats in the Xiaochengqi and Dachengqi groups were higher than those in the blank tumor group, especially in the Xiaochengqi group (P<0.01).The total tumor formation rate was 91.1%and 80%in the blank tumor groups , and 93.3%in both Xiaochengqi and Dachengqi groups , respectively .The average survival time of Xi-aochengqi group was lower than that of the blank tumor and Dachengqi groups ( P<0.01 and P<0.05 ) .The cumulative survival rate of the Xiaochengqi group and rats with a higher spleen-deficiency score was lower than that of the other groups (P<0.05).Conclusions Xiaochengqi decoction may induce spleen deficiency more seriously than Dachengqi decoction , and spleen deficiency may be an important unfavorable prognostic factor for rat models of liver cancer .
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Objective To study the therapeutic effect and mechanism of Jiawei Xiaochengqi Decoction on hepatic injures by endotoxin in rats. Methods Endotoxemia model of Wistar rats was made by thioacetamide. Blood biochemistry and pathological changes were examined to observe the effect of Jiawei Xiaochengqi decoction on liver function, TNF-?, endotoxin of the model rats. Results Jiawei Xiaochengqi Decoction could reduce the contents of TNF-?, endotoxin in the blood and the degree of hepatic cells necrosis of Wistar rats. There was significant difference compared with model group (P
ABSTRACT
Three Chengqi decoctions originated from Treatise on Cold Pathogenic Diseases refers to Tiaoweichengqi decoction,Xiaochengqi decoction and Dachengqi decoction.This article introduced clinical application of the three Chengqi decoctions for mental disease.This paper pointed out that treating for psychiatric patients with YangmingFushi syndrome with the corresponding decoction according to the degree of interior heat and excess can achieve satisfactory curative effects.Meanwhile,the author also put forward his own views on some problems that should be pay attention to in clinical application of the three Chengqi decoctions such as the dose of agents in decoction and the decocting methods.